Abstract: In the OFDM systems, the modulation symbols on the OFDM data subcarriers will seriously affect the parameter estimation of the single-tone interference when there are fewer OFDM symbols to be processed at a time. To solve this problem, this paper proposes a single-tone interference cancellation algorithm using frequency domain retrieval. This algorithm first uses DFT to estimate the single-tone interference frequency points, and then retrieves the modulation symbols of the subcarriers near the interfering frequency point to eliminate the influence on the main lobe of the single-tone interference in frequency domain. It improves the accuracy of interference parameters estimation and realizes accurate reconstruction and elimination of single-tone interference. The simulation results show that the new algorithm can effectively improve the estimation accuracy of the single-tone interference parameters and reduce the error rate of the OFDM systems.