Abstract: Aiming at the problem of the spreading codes blind estimation of the non-periodic long code direct-spreading CDMA signals(NPLC-DS-CDMA), firstly, the signals were received by the multi-antennas and the signals were constructed to matrix. Secondly, the singular value decomposition was performed on the received signal matrix, and the spread spectrum signal matrix of each user was estimated by FastICA algorithm from the extended singular value vector. Thirdly, the spread spectrum signal of each user was shifted and multiplied in order to remove the information codes, and then the shift sequences were obtained. Fourthly, the third order correlation function (TCF) of the sequence was calculated, and the correct TCF peaks were obtained by comparing the distance between the TCF’s estimate and the theoretical value at the coordinate point and its conjugate coordinate points. Finally, the primitive polynomial of the spreading code was obtained by using the matrix oblique elimination method. The simulation results verify the effectiveness of the proposed algorithm for the blind estimation of NPLC-DS-CDMA spread spectrum code.