Abstract: In the research of aircraft collision detection algorithm, one of the important problems is how to simulate conflict scenarios in a convenient and realistic method. A mutitarget track generation method based on general aviation aircraft motion model is presented in this paper. Firstly, the single target tracks of various flight stages should be described based on the general aviation aircrafts’ performance parameters and motion model. In order to make the target track be coherent and smooth, the cubic B-spline curve interpolation in the turning point of the track in each segmentis constructed. Secondly, refer to the definitions of the typical conflict scenarios in document DO-317 which is released by FAA, multi-target is generated and several conflict scenarios are designed to provide a theoretical basis for verification the conflict relief algorithm in each condition. Finally, put the simulation result into the ADS-B IN Application Software (AIAS), the results of the experiment in the software show the effectiveness of the collision detection algorithm and the simulation software is programmed by C#.