Abstract: This paper studied the signal reconstruction and system identification based on deconvolution algorithm in pulse metrology. The transition duration of pulse parameter national standard of China is 7.25±0.50ps, and bandwidth is 56.3±5.8%GHz. High speed sampling oscilloscope Agilent86100C has the bandwidth of 70GHz and minus step pulse source Picosecond4016 has the transition duration of 5ps, according to traditional calibration method, these two instruements could not be calibrated by the national standard. The deconvolution algorithm was used in the processing of measurement data in this paper, and conventional deconvolution method was improved to overcome the large errors while step signal was deconvoluted. Experiment verified the new algorithm, realized the value transfer.