Abstract: Cooperative communications is a reliable communication technology, by using spatial diversity, which can overcome channel fading, noise interference and path loss experienced by wireless communication transceivers, and effectively enhance the transmission performance of the system. At present, there are many basic theoretical researches on cooperative communication technology, but there is no real prototype system, and also no measured data as the theoretical basis. This paper designed and implemented an adaptive distributed cooperative transmission protocol, which could acquire channel quality information in real time to support adaptively relay selective and cooperative transmission. The principle prototype was independently developed, and the transmission performance of the system in indoor, outdoor, ground and air application scenarios was tested from three performance indicators of bit error rate, packet loss rate and throughput. The test results show that compared with the direct transmission mode, the adaptively distributed cooperative transmission technology proposed in this paper can effectively improve the reliable transmission performance and realize the autonomous cooperative communication network of distributed multiple nodes.